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Fig. 2. Anti-hFIX IgG antibodies in normal mice after protein injection or neonatal gene transfer. A. Anti-hFIX antibody levels after protein injections. Mice of the indicated strain that did not receive gene transfer began to receive weekly IP injections of 30 IU hFIX at 2 to months after birth, for a total of 10 doses. The relative levels of anti-hFIX IgG antibody in mg ml were determined by immunoassay and are plotted vs. the time in weeks after the first dose of protein. Each line represents a single animal. For the BALB c and C57BL 6 mice, 2 and 3 mice, respectively, failed to make.
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2.2 v -1.65 SD, P .42 ; . However, in patients treated with CY TBI, prior cranial irradiation was associated with growth rate impairment during the first year -2.6 SD v -0.9 SD, P .025 ; , but not in patients treated with BU CY P .70 ; Fig 3 ; . The occurrence of acute GVHD P .13 ; , and an older pretransplant age P .06 ; , were marginally associated with impaired growth rates during the first year. Gender P .38 ; , type of transplant P .99 ; , year of transplant P .62 ; , occurrence of chronic GVHD P .28 ; , number of prior treatments P .45 ; , and pretransplant height P S O ; were not associated with growth ratcs during thc first ycar after transplant. Multiple linear regression was used to determine which combination of these factors could best explain the variation in one-year growth rate Z scores. The potential interactions between diagnosis, cranial irradiation, and the number of prior treatment courses were evaluated, but none influenced the final regression model, except for a marginally significant interaction between diagnosis and cranial irradiation P .083 ; . Therefore, diagnosis, cranial irradiation, and thcir interaction were tested along with the other variables associated with growth rates with P values less than or equal to .I5 in the multivariate model. The independent factors identified by this multivariate analysis and the equations for first- and second-year growth rate Z scores generated by these multivariate models are given in Table 2.
PH ; , with an intersection point at pH 7.6, assigning a pKa value of MOXI. This is in accordance with literature data about reduction scheme of some fluoroquinolones antibiotic hydrogenation of the carboxylic group [19, 20]. For analytical purposes, 0.04 mol l-1 Phosphate buffer solution at pH 8.0 was therefore chosen as best work conditions. In continuity of the studies, the coordination number of the formed complex adsorbed on the electrode surface m ; and the stability constant of the complex ; was determined as described for Zhang et al [12, 13], through the use of the following equation.
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The amount and usefulness of information and 92% of the younger women complained of too little information and lack of support. In particular, we know that more patienttargeted technical information about modern tools to diagnosis would make a difference. The importance of supportive care cannot be over-estimated and it comes high on this agenda. Support for the psychological, emotional and practical wellbeing of the patient, her family and friends needs to start before diagnosis. Everyone connected with the patient will be touched to some degree by the wait for test results, the diagnosis, then the treatment and consequent changes in daily life. They, too, will experience fear of the unknown and may suffer inwardly, as they feel hopeless and helpless in the face of it all. Along with the patient, their needs must be addressed by the nurses, psychologists and counsellors who are vital members of the hospital multidisciplinary team. Better communication and shared responsibility with the patient's GP could help to improve continuity of care. Equally, peer support by recovered patients can be important in boosting hope and encouragement. They provide a role model for survival just when it is most needed and can bring great comfort to the patient and her family. In this context socially disadvantaged patients need special consideration as, for various reasons, they tend not to avail themselves of support services. When it comes to delivery of results, more than 5% of the CAWAC patients 600 in number ; had been informed of their diagnosis by telephone or letter. In a previous study by Loge and colleagues in 1997, 43% of patients had been informed by telephone, letter, in hospital corridors or on the ward round and similar anecdotal reports are not infrequent. This is hardly acceptable practice in imparting sensitive information and it seems that corrective attention is required in doctor-patient communication training. The manner in which results are.
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Academy of Sciences, with specialized scientific establishments, and with other enterprises and organizations. Al these organizations coordinate l on their research programmes, organize j i t investigatiton projects, convene scientific conferences and meetings, and publish the results of co-operative work. Research i financed by the state budget and through agres ements concluded by higher learning establishments with enterprises and organizations. The most important of the research and experimental n design projects which are elaborated are included i the research plans of the ministlries and departments under whose authority these establishments operate. Some projects wl find their w a y into the national ecoil nomic plans of the Republic. The research potential and oubput of Byelorussia's institutions of higher education have n o w reached such high levels that these institutions are regularly called upon to solve major scientific and technical problems which have a considerable bearing on the scientific and technical development OP the Republic. I addition, work on these problems serves n a a means of improving the training o highly skilled specialists. The s f research facilities available include two research institutes, a large number of experimental and specialized laboratories, co-operative higher education centres which grant technical asstistance for specific projects, specialized design offices, experimental grounds, and standardization and metrology services. More than 600 departments of one kind os another are actively conducting investigations. Thanks to these efforts the total research effort of Byeloruissia has s been growing constantly both as to volume and a to sophistication and profitability. During the years of the `10th5-year plan alone, the volume o research carried out i institutions of higher education inoreased by a f factolr of 1, 8.The share of major projects contracted i these institutions n went from 27, 8O, 'o to 54, 3% and has n o w reached the 70% mark. The n economic effect i the national economy of the use of the results of this research increased more than 3, 5 times. The effectiveness of this research work is also borne out by the publication o studies, textbooks, manuals, f n and the t housands of practical inventions resulting from it. It has i fact become a rule that scientists i hligher education take an active part n i the implementation of union-wide, national, special, and academic n research programmes. The leading research-oriented universities are the following : the Byelorussian, Gomel, and Grodno Universities, the Byelorussian Poly34 and medrol.
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Skeletal muscle Ca2 + -ATPase in 11 hyperthyroid patients before n 10 ; and after treatment euthyroid ; n 10 ; and in 8 healthy controls. Mean values with bars showing SD. P-values represent the comparison between patients in the hyperthyroid state and the euthyroid state using paired t-test n 9 ; and comparisons of healthy controls to patients in the hyperthyroid state or euthyroid state using unpaired t-test and megestrol!
| The length of hospital stay was 5 days for both of our study groups, whereas the Dutch study reports a statistically significant difference in hospital stay between the off-pump and on-pump groups, ie, 6 and 7 days, respectively. No significant difference in intubation time was found between our two study groups. Nevertheless, in an attempt to evaluate the effect of the OPCAB technique alone, we used standard intravenous fentanyl anesthesia for the two study groups. Much shorter intubation time could be achieved thanks to our experience gained with ultrafast track anesthesia [8], which we now use routinely. With this technique, more than 90% of patients undergoing OPCAB can be extubated in the operating room just after skin closure [9]. The use of the off-pump revascularization procedure coupled with thoracic epidural anesthesia without need for intubation also enables operation even in those patients whose impaired respiratory function precludes standard coronary artery surgery [10]. In agreement with another randomized study [11] we have demonstrated off-pump surgery to be significantly less costly than conventional surgery. A marked reduction of CK and CK-MB levels in the off-pump group is indicative of less damage to the myocardium by partial occlusion of coronary arteries compared with the global ischemia from complete cardiac arrest. Nevertheless, any effect on clinical outcome was not demonstrated. In agreement with most studies, we confirmed a reduced number of bypass grafts in the group undergoing surgery without CPB. Distribution of distal anastomoses among three coronary arteries is very similar in the two groups, but group B patients showed a tendency toward a more frequent location of anastomoses in the area of the left anterior descending artery and less frequent location in the area of the right coronary artery. The percentages of anastomoses to the circumflex artery branches were almost the same in both groups. The lower rate of bypasses per patient in our study seems to be related to the arterial size and quality rather than location. In the presence of a calcified coronary artery of insufficient diameter, the cardiac surgeon might be expected to refuse to make an anastomosis more frequently rather than to convert to CPB. Our early results did not show any impact of this fact on the clinical outcome. The clinical impact of lower numbers of grafts per patient will become apparent at longer-term follow-up. The population of patients undergoing surgical coronary revascularization has continued to become older and sicker [12]. These are exactly the types of patients who should benefit from OPCAB surgery [13]. In our study, the cardiac surgeon decided to change the technique in 5.4% of patients of each of the study groups before the operation. In 10 patients randomized into group A, the reasons for avoiding the use of CPB were the patient's age, indication for left anterior descending coronary artery single bypass, or the presence of multiple underlying diseases. Eleven younger group B patients needed the use of CPB because of planned multiple.
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P38-10 ANTIBODY INDUCED PERTURBATION A NEW METHOD TO IDENTIFY PATHWAYS IN BREAST CANCER PROGRESSION M. D. Johnson Georgetown University, Washington, DC P38-11 GENE AMPLIFICATION OF HER-2 NEU IN BREAST CANCER CELLS RESULTS IN ALTERED TETRASPANIN GENE EXPRESSION TO PROMOTE METASTATIC POTENTIAL D. P. Lombardi, 1 R. Ashfaq, 2 D. Tripathy, 2 J. Geradts, 3 M. S. Sinha, 1 K. T. Toal1 1 Washington University School of Medicine, St. Louis, MO; 2 University of Texas Southwestern Medical Center at Dallas, Dallas, TX; 3Roswell Park Cancer Institute, Buffalo, NY and memantine.
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Abbreviations used are as follows: abmr, autologous bone marrow rescue; abmt, autologous bone marrow transplantation; ccg, children's cancer group; gtr, gross total resection; mopp, mechlorethamine vincristine procarbazine prednisone; pfs, progression-free survival; pnet, primitive neuroectodermal tumor; pog, pediatric oncology group; seer, surveillance, epidemiology, and end results; ukccsg, united kingdom children's cancer study group and meperidine.
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ABSTRACT GH secretion is regulated by the interaction of GHRH and somatostatin and is released in 10 20 pulses in each 24-h cycle. The exact roles in pulse generation played by somatostatin, GHRH, and the recently isolated GH-releasing peptide, Ghrelin, are not fully elucidated. To investigate the GHRH-mediated GH secretion in human, we investigated pulsatile, entropic, and 24-h rhythmic GH secretion in two young adults male, 24 yr; female, 23 yr ; from a Moroccan family with a novel inactivating defect of the GHRH receptor gene. Data were compared with values in age- and gendermatched controls. Plasma GH concentration were measured by a sensitive immunofluorometric assay, with a detection limit of 0.01 mU L. All plasma GH concentrations in the female patient were measurable; in the male patient 30 of 145 samples were at or below the detection limit. GH secretion was pulsatile, with 21 and 23 secretory episodes 24 h in the male and female patients, respectively. The fraction of basal to total GH secretion was raised in both patients by 0.18 and 0.15, respectively. The total 24-h GH production rate was greatly diminished; in the male patient it was 6.9 mU L normal values for his age, 26 63 mU L ; , and in the female patient it was 4.2 mU L normal values for her age, 96 390 mU L ; . The nyctohemeral plasma GH rhythm was preserved P 0.001 ; , with normal acrophases 0430 and 0218 h in the male and female, respectively ; . Approximate entropy was greatly elevated in both subjects 0.82 in the male and 1.17 in the female; upper normal values for age and gender, 0.24 and 0.59, respectively ; . Intravenous injection of 50 g GHRH failed to increase the plasma GH concentration in both patients, but 100 g GH-releasing peptide-2 elicited a definite increase male patient, 0.13 to 1.74 mU L; female patient, 0.29 to 0.87 mU L ; . Both patients had a partial empty sella on magnetic resonance imaging scanning. In summary, the present studies in two patients with a profound loss of function mutation of the GHRH receptor favor the view that in the human the timing of GH pulses is primarily supervised by intermittent somatostatin withdrawal, and the amplitude of GH pulses is driven by GHRH. In addition, we infer that effectual GHRH input controls the GH cell mass and the orderliness of the secretory process. J Clin Endocrinol Metab 86: 2459 2464 and meclizine.
You know it's important, but other than having that piece of paper in the glove box of your truck, what is a Coggins test for anyway? We've taken a lot of questions on this topic, so don't feel alone if you're confused. The Coggins test is named after Dr. Leroy Coggins. The Call for appointments or with purpose is to test for the viral disease Equine Infectious Anemia EIA or Swamp general questions during office Fever ; . EIA can affect all equines but can not be transmitted to other animals or to humans. EIA is transmitted between equines by transmitting contaminated blood hours--8 to 5 PM. via fly bites or improper use of needles or equipment ; . What are the legal requirements for Coggins tests? NY state requires a negative Coggins test for all equines that are transported. For horses who live in and do not leave NY state, a test is valid for the calendar year in which it is drawn and the entire next calendar year. This causes a lot of confusion, so here's an example. Bob, the horse, has a negative Coggins test dated January 1, 2003. That test is valid through December 31, 2004. Bob's stablemate Joe has a Coggins test dated December 31, 2002 one day before "Most infected animals are Bob's ; . That test is valid through December 31, 2003 one year less than Bob's and mephenytoin.
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