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The fragmentation pathway for thiabendazole is shown in figure 4b with the protonated molecule, m z 202, fragmenting to give the m z 175, with the neutral loss of 27 da hcn.

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Soto, P. J., Beall, F. A., Nakamura, R. M., and Kupferberg, L. L.: Myocarditis in Rhesus Monkeys. Arch. Path. 78: 681 Dec. ; , 1964. Detailed blood chemistry and autopsy studies were performed on 20 apparently healthy rhesus monkeys that had recently been imported from India. In 18, there was histologic evidence of myocarditis. Neither viral inclusion bodies nor parasites were found in the heart. No bacteria were grown from heart cultures. Heart extracts did not kill suckling mice. Thus, the etiology of the myocarditis was not resolved Gupta has an thiabendazole sheaffer and thiotepa. 1. ALICATA, J.E. - Angiostrongylus cantonensis Nematoda: Metastrongylidae ; as a causative agent of eosinophilic meningitis of man in Hawaii and Tahiti. Canad. J. Zool., 40: 5, 1962. BADARO, R.; FALCOFF, F.; BADAR, F.S. et al. - Treatment of visceral leishmaniasis with pentavalent antimony and interferon gamma. New Engl. J. Med., 322: 16-21, 1990. CSPEDES, R.; SALAS, J.; MEKBEL, S. et al. - Granulomas entricos y linfticos con intensa eosinofilia tisular producidos por un strongildeo strongylata ; . Acta md. costarric., 10: 235-255, 1967. CHEN, H.T. - Un nouveau nematode pulmonaire, Pulmonema cantonensis n.g.n. sp. des rats de Canton. Ann. Parasit. hum. comp., 13: 312-317, 1935. CUCKLER, A.C.; EGERTON, J.R. & ALICATA, J.E. - Therapeutic effect of thiabendazole on Angiostrongylus cantonensis infection in rats. J. Parasit., 51: 392-396, 1964. DOENHOFF, M.J.J.; MODHA, J.R.; LAMBERTUCCI, J.R. & MCLAREN, D.J. - The immune dependence of chemotherapy. Parasit. today, 7: 16-18, 1991. HAWKING, F. - Diethylcarbamazine and new compounds for the treatment of filariasis. Adv. Pharmacol., 16: 129-194, 1979. HAYASHI, M. - Studies on chemotherapy of parasitic helminths. XXX ; . Clinical and pathological changes in mice infected with Angiostrongylus cantonensis and treatment with mebendazole and betamethasone. Jap. J. Parasit., 36: 80-87, 1987. HAYASHI, M; TERADA, M.; ISHII, A; KINO, H. & SANO, M. - Studies on chemotherapy of parasitic helminths. XVI ; . Anthelmintic effect of Mebendazole on Angiostrongylus cantonensis in rats. Jap. J. Parasit., 31: 575-580, 1982. HWANG, K.P. & CHEN, E.R. - Larvicidal effect of albendazole against Angiostrongylus cantonensis in mice. Amer. J. trop. Med. Hyg., 39: 191-195, 1988. ISHII, A. - Effect of the anthelmintic levamisole on the first-stage of Angiostrongylus cantonensis in infected rats. Parasit. Res., 80: 267-270, 1994. ISHII, A.I.; TERADA, M.; FUJIU, Y. & SANO, M. - In vivo efficacy of levamisole against larval stages of Angiostrongylus cantonensis and Angiostrongylus costaricensis. Southeast Asian J. trop. Med. publ. Hlth., 20: 109-117, 1989. ISHII, A.I.; TERADA, M.; KINO, H.; HAYASHI, M. & SANO, M. - Studies on chemotherapy of parasitic helminths: effects of avermectin B1a on Angiostrongylus cantonensis in rats. Int. J. Parasit., 13: 491-498, 1983. ISHII, A.I.; TERADA, M. & SANO, M. - Studies on chemotherapy of parasitic helminths. XXIII ; . Effects of ivermectin on Angiostrongylus cantonensis in rats. Jap. J. Parasit., 34: 411-417, 1985. ISHII, A.I.; TERADA, M. & SANO, M. - Motility and drug susceptibility of Angiostrongylus cantonensis developing from gamma-irradiated first-stage larvae. Southeast Asian J. trop. Med. publ. Hlth., 18: 547-551, 1987. ISHIH, A.; YANOH, M.; IKEYA, C.; BAN, A. & TERADA, M. - Effects of anthelmintics on the development of eggs of Angiostrongylus costaricensis in vitro. J. Helminth., 75: 351-354, 2001. JOUBERT, J.; JOUBERT, M.J. & LOMBAARD, C.M. - Neurocysticercosis: a comprehensive approach to medical treatment. S. Afr. med. J., 68: 11-14, 1985.

TABLE 1. Cohorts selected from the source population of singleton children bom In Uppsala, Sweden, In 1973-1977, with parents who were living In Uppsala and had been bom In Sweden n 9, 808 ; , using Information from the Medical Birth Registry and the Inpatient Registry. Corresponding Author: F.Roelfsema, Department of Metabolism and Endocrinology, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands, Fax: + 31 71 5248136, email: f.roelfsema lumc.nl and thorazine. CLEAN-UP PROCEDURES The required efficiency of the clean-up procedure depends largely on the specificity and sensitivity of detection in the procedure selected for determination. Obviously, the least demanding procedures with regard to the efficacy of clean-up are bioassay methods on thin layer plates. The extracts can be spotted onto the plates without further clean-up and can be developed Fernandes and Cole 29 Wegman et al. 90 Baker and Hoodless 6 Cox and Pinegar 22 Ben Arie 11 Ellis and Sinclair 26 Homans and Fuchs 38 . For all other methods of determination a more or less rigorous clean-up is necessary. The weakly basic property of the N-atom in the benzimidazoles can be utilized for an efficient partition step in the clean--up procedure. The residue dissolved in ethyl acetate or benzene, Rouchaud and Decallonne 65 ; or chloroform, Rajzman 64 can be extracted very easily with diluted hydrochloric acid and reextracted into ethyl acetate Chloroform ; upon adjusting the acid aqueous phase back to pH 8 - Sims et al. 72 Cmx and Pinegar 22 Kirkland et al. 41 Aharonson and Ben Aziz 1 Austin et al. 2 Rouchaud and Decallone 65 Rouchaud et al. 66 Tanaka and Fujimoto 80 or into chloroform after adjusting the aqueous phase to pH 6.5. After this clean-up step, the residue carbendazim ; can be reextracted with 0, 1 M MCi and determined, e.g. by UV in the aqueous solution see under section Colorinetric and Spectrophotometric Methods. TLC was incorporated as a clean--up step in the UV--spectrophotometric determination by White and Kilgore 91 ; to eliminate interfering substances. For the separation of carbendazim from plant and other materials, column chromatography has been used in several cases. Florisil was used by Douch 25 ; A combination of two adsorbents; an upper layer of magnesium oxide "Celite" 1 + 6 ; and a lower one of alumina grade III ; was used by Aharonson and Ben Aziz 1 ; . Benomyl is converted to carbendazin in this column by the magnesium oxide "Celite" layer. Thiabendazole is subsequently eluted with ethyl acetate and carbendazim with ethanol ethyl acetate 1 + 1 ; was found that interfering fluorescent compounds were eliminated. Neutral alumina was used for the column chronatographic clean--up by Watkins 88 ; and alumina with 6 % water and chloroform or ethyl acetate as elution solvent by Cox and Pinegar 22 ; . Siegel 71 ; used the cation exchange Dowex 50 x 8 -- 200 resin in a column to separate carbendazim from interfering substances. The elution step was done in the batch mode using 4 N NH4OH as eluant. Thin layer chromatography and high speed liquid chromatography are separation techniques or clean--up procedures ; , but they are usually regarded as detection determination procedures and will, therfore, be discussed in the following section. DETECTION AND DETERMINATION Bioassay For the detection of the benzimidazoles, fungal toxicity assays have been used by a number of authors. The assay is carried out, in practically all case; after thin--layer chromatography of the test solution. The plates are coated with an appropriate nutrient solution and sprayed with a suspension of the test organisms. The inhibition zones developing after maintainance of appropriate cultivation conditions for a given time are in some instances correlated to the fungicide concentrations. A summary of the test species used are given in the table No. 6.

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